Digital confocal microscopy through a multimode fiber

摘要: Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy an important tool for biomedical imaging since it improves image quality by rejecting background signals. However, suffers from low sensitivity in due to light scattering. Recently, multimode fibers have provided new paradigm minimally invasive endoscopic controlling propagation through them. Here we introduce combined technique where confocal are acquired fiber. We achieve this digitally engineering the excitation wavefront and then applying virtual digital pinhole on collected signal. In way, able acquire fiber with significantly increased contrast. With of numerical aperture 0.22, lateral resolution 1.5µm, axial 12.7µm. The point-scanning rate currently limited our spatial modulator (20Hz).