The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species

摘要: Abstract Introduction Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-toff MS) is a reliable method for diagnosing number bacterial and fungal infections. It also effective as rapid diagnosis several parasitic agents. We used MALDI-toff MS to study the protein profiles four nematodes: Dirofilaria repens, Dirofilaria. immitis, Ascaris suum lumbricoides. Methods studied dirofilaria (five each species: D. repens immitis) ascaris A. lumbricoides), using proteomic analysis based on MS. Results Analysis extracts showed spectra with high-intensity peaks in range 2–20 kDa. The quality (clear graphical reflection mass/charge luminous intensity, consistent repeated analyzes) intensity spectral were all samples same species. immitis differed eight major which makes it possible differentiate species according profile. obtained from lumbricoides proteins slightly 3 both discovered m/z 13000; 13400 14400. diapason 3000 kD–7300 kD specific genus are constant. Conclusions MS-based can serve an taxonomic tool parasitological studies.