Advanced Technology for Gene Delivery with Homing Peptides to Spinal Cord through Systemic Circulation in Mice.
作者： Tomoya Terashima , Nobuhiro Ogawa , Toshiyuki Sato , Miwako Katagi , Yuki Nakae
DOI: 10.1016/J.OMTM.2019.04.008
关键词:
摘要: Homing peptides to the spinal cord were identified and isolated using phage display technology. In vivo biopanning was performed by intravenous systemic injection of a library screen specific targeting mice. Analyses of sequences targeted phages yielded two candidate cord: SP1 (C-LHQSPHI-C) SP2 (C-PTNNPRS-C). These synthesized intravenously injected into mice evaluate their tissue specificity potential as gene delivery carriers. The complexes between or plasmid vector expressing reporter could induce transduction in through without expression brain, liver, kidney. In addition, injection of complex vectors induced interleukin-4 cord, resulting effective suppression lipopolysaccharide-induced hyperalgesia. Therefore, administered homing complexed with provided tissue-specific treatment featuring CNS circulation. This novel method is feasible has great for clinical application.
sci-hub.se 参考文章(37)
Shuanglin Hao, Marina Mata, Joseph C Glorioso, David J Fink, HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain. Molecular Pain. ,vol. 2, pp. 6- 6 ,(2006) , 10.1186/1744-8069-2-6
Anders Wittrup, Angela Ai, Xing Liu, Peter Hamar, Radiana Trifonova, Klaus Charisse, Muthiah Manoharan, Tomas Kirchhausen, Judy Lieberman, Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown Nature Biotechnology. ,vol. 33, pp. 870- 876 ,(2015) , 10.1038/NBT.3298
Héctor R Méndez-Gómez, Albert Galera-Prat, Craig Meyers, Weijun Chen, Jasbir Singh, Mariano Carrión-Vázquez, Nicholas Muzyczka, USA Florida, Transcytosis in the blood–cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system Molecular therapy. Methods & clinical development. ,vol. 2, pp. 15037- 15037 ,(2015) , 10.1038/MTM.2015.37
O N Hausmann, Post-traumatic inflammation following spinal cord injury. Spinal Cord. ,vol. 41, pp. 369- 378 ,(2003) , 10.1038/SJ.SC.3101483
Kathleen Beusterien, Jessica Grinspan, Iryna Kuchuk, Sasha Mazzarello, Susan Dent, Stan Gertler, Nathaniel Bouganim, Lisa Vandermeer, Mark Clemons, Use of Conjoint Analysis to Assess Breast Cancer Patient Preferences for Chemotherapy Side Effects Oncologist. ,vol. 19, pp. 127- 134 ,(2014) , 10.1634/THEONCOLOGIST.2013-0359
V Dietz, Spasticity-spastic movement disorder Spinal Cord. ,vol. 46, pp. 588- 588 ,(2008) , 10.1038/SC.2008.45
Rosemary Kanasty, Joseph Robert Dorkin, Arturo Vegas, Daniel Anderson, Delivery materials for siRNA therapeutics Nature Materials. ,vol. 12, pp. 967- 977 ,(2013) , 10.1038/NMAT3765
Hongtao Lv, Shubiao Zhang, Bing Wang, Shaohui Cui, Jie Yan, Toxicity of cationic lipids and cationic polymers in gene delivery. Journal of Controlled Release. ,vol. 114, pp. 100- 109 ,(2006) , 10.1016/J.JCONREL.2006.04.014
Renata Pasqualini, Erkki Koivunen, Erkki Ruoslahti, αv Integrins as receptors for tumor targeting by circulating ligands Nature Biotechnology. ,vol. 15, pp. 542- 546 ,(1997) , 10.1038/NBT0697-542
Mikhail Kolonin, Renata Pasqualini, Wadih Arap, Molecular addresses in blood vessels as targets for therapy. Current Opinion in Chemical Biology. ,vol. 5, pp. 308- 313 ,(2001) , 10.1016/S1367-5931(00)00207-6