Transcytosis in the blood–cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system

摘要: Crossing the blood–brain and blood–cerebrospinal fluid barriers (BCSFB) is one of fundamental challenges in development new therapeutic molecules for brain disorders because these prevent entry most drugs from blood into brain. However, some large molecules, like protein transferrin, cross using a specific receptor that transports them Based on this mechanism, we engineered receptor/ligand system to overcome by combining human transferrin with cohesin domain Clostridium thermocellum, tested hybrid choroid plexus mouse dockerin ligand. By expressing our choroidal ependymocytes, which are part BCSFB, found systemically administrated ligand was able bind accumulate where transported side side.