Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1.

摘要: Previous studies have suggested that murine interleukin 1 (IL 1) may be synthesized as a high m.w. precursor. Using specific antibodies against IL 1, we analyzed the primary form of by normal peritoneal macrophages and P388D1 cell line macrophages, in vitro using poly (A)+ RNA from stimulated macrophages. In all cases, labeled protein immunoprecipitated with anti-IL exhibited 33,000 on SDS gels. This was not an aggregate low 1. Addition excess purified completely blocked immunoprecipitation protein. When cells were pulsed [35S]methionine for to 5 hr then incubated medium containing unlabeled methionine 19 hr, detected culture fluid. If label presence maximally effective concentration synthesis inhibitor, cycloheximide, still found Our results indicate is precursor converted fluid