In Vitro Studies on the Effect of Erythropoietin on Glucosamine-l-14C Incorporation Into Rat Bone Marrow Cells

摘要: It has been shown that glucosamine-l-14C is incorporated into the stroma (acid-insoluble fraction) of rat bone marrow cells in culture. Erythropoietin preparations at stages purification ranging from untreated anemic plasma to one highly purified material (approximately 900 U/mg protein) caused increased glucosamine uptake these cell cultures. therefore assumed erythropoietin agent responsible for this effect, which first becomes measurable after about 3 hr incubation. A fixed amount incorporating capacity appears added The response a logarithmic function dose over considerable part range tested. radioactivity identified classes compounds: a) not solubilized by 0.05N H2SO4 80 C (44% total original sample); b) so far stayed origin paper-chromatographic system ...