Swim Stress differentially blocks CRF receptor mediated responses in dorsal raphe nucleus

摘要: Summary Modulation of the serotonergic (5-HT) neurotransmitter system arising from dorsal raphe nucleus (DR) is thought to support behavioral effects swim stress, i.e., immobility. In vivo pharmacological and anatomical studies suggest that corticotropin-releasing factor (CRF) γ-aminobutyric acid (GABA) synaptic transmission closely interact set response DR stress. To investigate cellular basis these physiological mechanisms ovine CRF (oCRF) on GABA A -dependent miniature inhibitory postsynaptic currents (mIPSCs) in 5-HT non-5-HT neurons acute mesencephalic slices obtained rats either naive or 24 h after a 15 min stress session were tested. this study, effect alone was decrease holding current, hyperpolarize neuron, increase amplitude charge mIPSCs recorded neurons. Ovine (10 nM) induced an mIPSC frequency rats, suppressed by The inward current elicited oCRF both also blocked increased neurons, but not modified concert with our previous findings decreased input resistance, action potential threshold duration glutamatergic activity overall primary excitability These data provide mechanism at level for immobility identifies critical components circuitry responsible altered output