Interaction between microRNA-181a and TNFAIP1 regulates pancreatic cancer proliferation and migration.
作者： Peng Zhang , Zhiyong Guo , Ronglin Hu , Xiaoshun He , Xingyuan Jiao
DOI: 10.1007/S13277-015-3704-8
关键词:
摘要: We investigated the role of microRNA 181a (miR-181a) and its downstream target tumor necrosis factor, alpha-induced protein 1 (TNFAIP1) in pancreatic cancer regulation. Quantitative real-time PCR (qRT-PCR) was applied to evaluate gene expression miR-181a seven cell lines. MiR-181a inhibitor lentivirus (miR-181a-IN) used down-regulate Capan-1 AsPC-1 cells. The effects down-regulation on were evaluated by vitro proliferation assay migration assay. Targeting TNFAIP1 dual-luciferase reporter western blot. either upregulated pcDNA3.1 (+) vector or down-regulated siRNA subsequent upregulation mediated regulation also through assays. vivo effect growth a xenograft consistently inhibited vitro, Ectopic overexpression had similar tumor-suppressive as down-regulation, whereas siRNA-mediated opposite oncogenic cancer. In showed recapitulated anti-tumor TNFAIP1. played critical regulating migration, likely interacting with
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