MiR-148a impairs Ras/ERK signaling in B lymphocytes by targeting SOS proteins.

摘要: // Julia Alles 1 , Nicole Ludwig Stefanie Rheinheimer Petra Leidinger Friedrich A. Grasser 2 Andreas Keller 3 and Eckart Meese Institute of Human Genetics, Saarland University, 66421 Homburg, Germany Virology, Chair for Clinical Bioinformatics, 66123 Saarbrucken, Correspondence to: Alles, email: ju.al@mx.uni-saarland.de Keywords: B cells, microRNA, miR-148a, SOS, ERK Received: March 15, 2017      Accepted: April 24, Published: May 07, 2017 ABSTRACT Although microRNAs have been recognized as central cellular regulators, there is an evident lack knowledge about their targets. Here, we analyzed potential target genes miR-148a functioning in Ras signaling including SOS1 SOS2. A dual-luciferase reporter assay showed significantly decreased luciferase activity upon ectopic overexpression HEK-293T cells that were co-transfected with the 3′UTR either or Each 3′UTRs SOS2 contained two binding sites both which necessary activity. MiR-148a lead to reduced levels endogenous proteins. Likewise, SOS proteins found cell lines transfected miR-148a. The level ERK1/2 phosphorylation one most relevant downstream members Ras/ERK pathway was also overexpression. data show impairs via cells.