Anti-troponin-T monoclonal antibody crossreacts with all muscle types
作者： Soo-Siang Lim , Zeng-Hong TU , Larry F. Lemanski
DOI: 10.1007/BF00713258
关键词:
摘要: Monoclonal antibodies to troponin-T were produced by the hybridoma technique. Culture supernatants initially screened using an enzyme-linked immunoabsorbent assay (ELISA). Positive clones subcloned twice and further characterized. One of these, 7/H3:C9:D10, against troponin-T; immunoblotting experiments indicated its specificity for only when challenged with a variety striated muscle myofibrillar proteins. Indirect immunofluorescence staining antibody shows specific I-band in both adult embryonic skeletal cardiac various vertebrate species. In addition, intense but diffuse cytoplasmic was seen chicken gizzard smooth muscle. Our results suggest that contains antigenic determinant is common
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