The Carboxyl-Terminal Region Is a Determinant for the Intracellular Behavior of the Chorionic Gonadotropin β Subunit: Effects on the Processing of the Asn-Linked Oligosaccharides
作者： Mesut Muyan , Irving Boime
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摘要: The placental hormone human CG (hCG) consists of two noncovalently linked α- and β-subunits similar to the other glycoprotein hormones LH, FSH, TSH. These heterodimers share a common α subunit but differ in their structurally distinct β subunits. CGβ is distinguished among subunits by presence C-terminal extension with four serine-linked oligosaccharides (carboxyl terminal peptide or CTP). In previous studies we observed that deleting this sequence decreased assembly truncated (CGβ114) α-subunit increased heterogeneity secreted forms uncombined synthesized transfected Chinese hamster ovary (CHO) cells. latter result was attributed alterations processing N-linked oligosaccharides. To examine at what step occurs, CGβ114 genes were into wild-type mutant CHO cell lines are defective late steps carbohydrate-processing pat...
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