Possible involvement of C5/C5a in the efferent and elicitation phases of contact sensitivity.

摘要: The elicitation of 24-h contact sensitivity (CS) in mice requires a serotonin-dependent, 2-h response called CS initiation. We studied the role complement (C) by comparing DBA/1 (C5-normal) vs DBA/2 (C5-deficient) and found impaired 2-h, but not 24-h, CS. showed previously that responses represent initiation are required for responses. Treatment C5-deficient with absent macroscopic (ear swelling) selective serotonin antagonist inhibited CS, suggesting had submacroscopic (i.e., microscopic), serotonin-dependent When normal mouse serum was used as source C5 to reconstitute mice, significant were restored. Furthermore, heat treatment inactivate C abrogated restoration Thus, suggested be involved Using suboptimal immunizing dose Ag revealed an component also B10.D2/o compared C5-normal B10.D2/n eliciting Ag. Again, reconstitution restored deficient classical probably depend part on C5. These results imply may play via preceding