Antagonism of myotoxic and paralyzing activities of bothropstoxin-I by suramin.
作者： Maristela de Oliveira , Walter LG Cavalcante , Emerson Z Arruda , Paulo A Melo , Maeli Dal-Pai Silva
DOI: 10.1016/S0041-0101(03)00166-1
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摘要: Abstract Polyanionic substances are known to inhibit the myotoxic effects of some crotalide snake venoms. Bothropstoxin-I (BthTX-I), a basic Lys49 phospholipase (PLA2) homologue from Bothrops jararacussu venom, besides inducing muscle damage, also promotes blockade both directly and indirectly evoked contractions in mouse neuromuscular preparation. In this work, we evaluated ability suramin, polysulfonated naphtylurea derivative, antagonize paralyzing activities BthTX-I on mice junction vitro. Myotoxicity was assessed by light electronic microscopic analysis extensor digitorum longus (EDL) muscles; activity through recording phrenic-diaphragm (PD) preparations. (1 μM) alone, or pre-incubated with suramin (10 at 37 °C for 15 min added preparations 120 min. induced histological alterations typical myonecrosis 14.6±1.0% EDL fibers. addition, blocked 50% PD 72.1±9.1 21.1±2.0 min, respectively. Pre-incubation abolished muscle-damaging muscle-paralyzing BthTX-I. Since is polyanionic substance, suggested that its result formation inactive acid–base complexes
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