Calcium Channel Blockers as Inhibitors of Angiotensin I–Converting Enzyme
作者： Dulce E. Casarini , Adriana K. Carmona , Frida L. Plavnik , Maria T. Zanella , Luiz Juliano
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摘要: Using ion-exchange chromatography of dialyzed human urine from healthy and hypertensive patients, we detected two peaks angiotensin I-converting enzyme (ACE) activity on hippuryl-His-Leu eluted at ionic strengths 0.7 (F1 peak) 1.25 (F2 mS. These hydrolytic activities decreased gradually in the patients submitted to isradipine treatment, F2 F1 disappearing after 12 24 hours, respectively. By Western blot analysis, fractions corresponding both untreated presenting ACE treated (24 hours) without this were recognized by an ACE-specific antibody. results indicated that was present but inhibited isradipine-treated patients. In vitro assays with isolated guinea pig plasma demonstrated is other commercially available calcium channel blockers, such as felodipine, nifedipine, verapamil. A noncompetitive inhibition observed all blockers studied. conclusion, these suggest besides primary effect channels, more commonly used are also inhibitors. The development efficient higher inhibitory could result interesting bifunctional antihypertensive drugs.
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