Role of xanthine oxidoreductase in experimental acute renal-allograft rejection.

摘要: BACKGROUND Increased oxygen radical production may not only contribute to posttransplant ischemia-reperfusion injury but also acute rejection of renal allografts. Xanthine oxidoreductase (XOR) constitute a relevant reactive species (ROS) source. The study was conducted (1). determine ROS as well oxidant and antioxidant enzyme activities in grafts (2). modulate by tungsten administration, specific inhibitor XOR. METHODS Syngraft (Lewis Lewis, Fisher344 Fisher344) allograft (Fisher344 Lewis) kidney transplantations were performed with or without administration. Analysis at day 1, 3, 9 posttransplantation. RESULTS Generation enhanced, being 10-fold higher allografts versus control kidneys (P <0.01); this associated histologic signs rejection. Oxygen radicals generated significant degree enhanced XOR activity, which increased more than posttransplantation; protein glomeruli tubulointerstitium elevated allo-grafts. In addition, NADPH oxidase activity significantly enzymes tended decrease. Tungsten treatment resulted pronounced reduction production, any effect on NADPH-oxidase activity; mononuclear cell infiltration ameliorated post-transplantation selective inhibition CONCLUSIONS A major part generation contributed are because alleviated phenomena.