Isotretinoin ameliorates renal damage in experimental acute renal allograft rejection.

摘要: BACKGROUND Retinoic acids, derivatives of vitamin A, act through retinoid receptors that are expressed in renal and immunocompetent cells (B T cells; monocytes macrophages). In experimental models glomerulonephritis interstitial disease, retinoids were shown to reduce both glomerular tubular damage inflammation. We therefore examined whether cellular rejection a model acute allograft rejection. METHODS Kidneys Fisher rats (F344, RT11v1) orthotopically grafted Lewis (RT11). Animals killed 7 or 14 days after transplantation. Rats undergoing transplantation treated with isotretinoin (13 cis-retinoic acid) at low dose 2 mg/kg body weight per day (LD isotretinoin) high 20 (HD vehicle. RESULTS At 14, albuminuria was reduced by approximately 70% (vehicle: 1.1+/-0.2 mg/24 hr vs. LD isotretinoin: 0.32+/-0.1 hr; P<0.001). serum creatinine levels significantly higher the vehicle-treated group than HD isotretinoin-treated (P<0.05). Both vascular injury compared (score 14: 20.1+/-5.1 vehicle 57.8+/-9.9, P<0.01), (score: 6.8+/-1.0 10.6+/-0.9 P<0.05), number macrophages cytotoxic cells. Isotretinoin also lessened tubulointerstitial damage, cell proliferation, infiltrating tubulointerstitium. CONCLUSIONS ameliorated functional, vascular, glomerular, lesions graft Although current study did not definitely eliminate possibility only delayed process, retinoic acid may provide new approach treatment injury.