An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy.
作者： John Fuesler , Yasunori Nagahama , Joseph Szulewski , Joshua Mundorff , Stephanie Bireley
DOI: 10.1016/J.GENE.2012.01.011
关键词:
摘要: The various iterations of the HapMap Project and many genome-wide association studies (GWAS) have identified hundreds potential genes involved in monogenic multifactorial traits. We constructed an arrayed 115,000-member human genomic library PAC shuttle vector pJCPAC-Mam2 that can be propagated both bacterial cells. appears to represent a two-fold coverage genome. Transient transfection p53-containing clone into p53-null Saos-2 osteosarcoma cells demonstrated p53 mRNA protein were produced. Additionally, expression triggered apoptosis subset This should serve as valuable resource validate disease by GWAS cell lines animal models. Also, individual members could potentially used for gene therapy trials variety recessive disorders.
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