Construction of bacterial artificial chromosome (BAC/PAC) libraries.
作者： Kazutoyo Osoegawa , Pieter J. Jong , Eirik Frengen , Panayiotis A. Ioannou
DOI: 10.1002/0471142727.MB0509S55
关键词: Cloning 、 Biology 、 Gel electrophoresis 、 Bacterial artificial chromosome 、 Genomic library 、 DNA 、 EcoRI 、 genomic DNA 、 Molecular biology 、 DNA sequencing
摘要: Large-insert genomic libraries are necessary for physical mapping of large chromosomal regions, isolation complete genes, and use as intermediates in DNA sequencing entire genomes. Construction BAC PAC is detailed the unit, including preparation or vector cloning by digestion with BamHI EcoRI, dephosphorylation alkaline phosphatase, purification through pulsed-field gel electrophoresis (PFGE). For high-molecular weight cloning, procedures embedding total from lymphocytes animal tissue cells also provided. Other protocols detail partial MboI a combination EcoRI endonuclease methylase (including methods optimizing extent digestion), subsequent size fractionation preparative PFGE. Finally, plasmid analyzing clones presented.
sci-hub.se 参考文章(24)
F P Gibson, D R Leach, R G Lloyd, Identification of sbcD mutations as cosuppressors of recBC that allow propagation of DNA palindromes in Escherichia coli K-12. Journal of Bacteriology. ,vol. 174, pp. 1222- 1228 ,(1992) , 10.1128/JB.174.4.1222-1228.1992
Guo‐Liang Wang, Thomas E Holsten, Wen‐Yuan Song, He‐Ping Wang, Pamela C Ronald, None, Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus. Plant Journal. ,vol. 7, pp. 525- 533 ,(1995) , 10.1046/J.1365-313X.1995.7030525.X
G Chu, D Vollrath, R. Davis, Separation of large DNA molecules by contour-clamped homogeneous electric fields Science. ,vol. 234, pp. 1582- 1585 ,(1986) , 10.1126/SCIENCE.3538420
H. Shizuya, B. Birren, U. J. Kim, V. Mancino, T. Slepak, Y. Tachiiri, M. Simon, Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 8794- 8797 ,(1992) , 10.1073/PNAS.89.18.8794
J. C. Pierce, B. Sauer, N. Sternberg, A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 2056- 2060 ,(1992) , 10.1073/PNAS.89.6.2056
Kazutoyo Osoegawa, Peng Yeong Woon, Baohui Zhao, Eirik Frengen, Minako Tateno, Joseph J. Catanese, Pieter J. de Jong, An Improved Approach for Construction of Bacterial Artificial Chromosome Libraries Genomics. ,vol. 52, pp. 1- 8 ,(1998) , 10.1006/GENO.1998.5423
S. Fukushige, B. Sauer, Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 7905- 7909 ,(1992) , 10.1073/PNAS.89.17.7905
Min Wang, Xiao-Ning Chen, Stephanie Shouse, Jim Manson, Qingzhou Wu, Rumei Li, Janet Wrestler, David Noya, Zhi-Guang Sun, Julie Korenberg, Eric Lai, Construction and Characterization of a Human Chromosome 2-Specific BAC Library Genomics. ,vol. 24, pp. 527- 534 ,(1994) , 10.1006/GENO.1994.1662
Sung-Sick Woo, Jiming Jiang, Bikram S. Gill, Andrew H. Paterson, Rod A. Wing, Construction and characterization of bacterial artificial chromosome library ofSorghum bicolor Nucleic Acids Research. ,vol. 22, pp. 4922- 4931 ,(1994) , 10.1093/NAR/22.23.4922
YuLing Sheng, Valeria Mancino, Bruce Birren, Transformation of Escherichia coli with large DNA molecules by electroporation Nucleic Acids Research. ,vol. 23, pp. 1990- 1996 ,(1995) , 10.1093/NAR/23.11.1990