BAC library construction, screening and clone sequencing of lake whitefish (Coregonus clupeaformis, Salmonidae) towards the elucidation of adaptive species divergence.

摘要: Genomic DNA sequences and other genomic resources are essential towards the elucidation of bases adaptive divergence reproductive isolation. Here, we describe construction, characterization screening a nonarrayed BAC library for lake whitefish (Coregonus clupeaformis). We then show how combined use next-generation sequencing can lead to efficient full-length assembly candidate genes. The consists 181,050 clones derived from single heterozygous fish. mean insert size is 92 Kb, representing 5.2 haploid genome equivalents. Ten were isolated following quantitative real-time PCR approach that targeted five previously identified Sequencing these on 454 GS FLX system yielded 178,000 reads with length 358 bp, total 63.8 Mb. De novo annotation allowed retrieval contigs corresponding each gene, which also contained up- and/or downstream noncoding sequences. These results suggest technologies will be key achieve better understanding both isolation in species pairs as well salmonid evolution general.