The abalone egg vitelline envelope receptor for sperm lysin is a giant multivalent molecule
作者： W. J. Swanson , V. D. Vacquier
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摘要: Abalone sperm lysin is a 16-kDa acrosomal protein, which nonenzymatically and species selectively creates hole in the egg vitelline envelope (VE) through passes to reach cell membrane. The crystal structures of both monomeric dimeric lysins have been solved sequences from 20 abalone determined. As first step understanding molecular mechanism by VE, its VE receptor was isolated. for (VERL) an unbranched, rod-like molecule with approximate relative mass 2 million; half being carbohydrate. Fluorescence polarization studies showed positive cooperativity binding VERL (EC50 ≈9 nM) were consistent selectivity dissolving VEs. Each bound between 126 142 molecules (two independent assays), showing that possesses repetitive lysin-binding motifs.
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