Porous chitosan scaffold cross-linked by chemical and natural procedure applied to investigate cell regeneration
作者： Chih-Kai Yao , Jiunn-Der Liao , Chia-Wei Chung , Wei-I. Sung , Nai-Jen Chang
DOI: 10.1016/J.APSUSC.2012.05.128
关键词:
摘要: Abstract Porous chitosan scaffold is used for tissue engineering and drug delivery, but limited as a material due to its mechanical weakness, which restrains cell adhesion on the surface. In this study, chemical reagent (citrate) natural (genipin) are cross-linkers formation of chitosan-based films. Nanoindentation technique with continuous stiffness measurement system particularly applied porous surface examine characteristic modulus nanohardness The genipin-cross-linked ≈2.325 GPa, significantly higher than that an uncross-linked one (≈1.292 GPa). cell-scaffold interaction assessed. morphology results MTS assay 3T3-fibroblast cells indicate enhancement properties induced proliferation modified pore size film can be tuned specific applications such regeneration.
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