Multiple restriction fragment length polymorphisms of the human epidermal growth factor receptor gene.

摘要: Abstract We have examined the epidermal growth factor (EGF) receptor gene for structural alterations in fresh human tumors. DNA samples from 92 patients with solid tumors (lung cancer, 37; breast 24; head and neck 17; other tumors, 14) were analyzed compared those 22 leukemia 14 individuals without malignant neoplasms. When digested Hin dIII restriction endonuclease, Southern blot analysis demonstrated 3 distinct polymorphic bands (9.8, 11, 12 kilobases) after hybridization to HER-A64-1 probe another 2 (4.9 5.2 HER-A64-3 probe. Pedigree of 43 members a single family comparative tumor normal same that variations fragment size observed due independent length polymorphisms region EGF gene. Amplification was detected cases but not studied. conclude has multiple rearrangement amplification occur infrequently, if ever, within encompassed by complementary probes used.