Monomolecular Layer of the Alkylated Cytochrome b562 Formed at the Air/Water Interface
作者： Kazutoshi Kobayashi , Teruyuki Nagamune , Taiji Furuno , Hiroyuki Sasabe , None
DOI: 10.1246/BCSJ.72.691
关键词:
摘要: A method is provided for forming a monomolecular layer of cytochrome b562 at the air/water interface by introducing dialkyl chains onto water-soluble b562. The purpose present study was to form densely and/or regularly packed two-dimensional arrays protein molecules. Protoheme, which prosthetic group b562, extracted from and then dialkylated(dioctadecylated) with synthetic-chemical procedure. This dioctadecylated heme reconstituted apo-cytochrome in dimethyl sulfoxide–water mixtures vitro. reconstitution reaction successful when time greater than 50 min, sulfoxide content 63% temperature maintained 10 °C. Surface pressure–area per molecule isotherm holo-cytochrome measured this clearly shows monolayer formation on water subphase. coefficient compressibility fi...
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