Monolayer Formation of Cytochrome b562 on Gold Surfaces and Its Reconstitution Reaction, Studied by Surface Plasmon Resonance Spectroscopy
作者： Kazutoshi Kobayashi , Masafumi Shimizu , Teruyuki Nagamune , Hiroyuki Sasabe , Yu Fang
DOI: 10.1246/BCSJ.75.1707
关键词: Heme 、 Cytochrome 、 Moiety 、 Analytical chemistry 、 Dissociation (chemistry) 、 Guanidine 、 Monolayer 、 Adsorption 、 Photochemistry 、 Molecule 、 Chemistry
摘要: Association and dissociation reactions of cytochrome b562 on solid surfaces were investigated by means surface plasmon resonance spectroscopy. Propionates side chains in the heme molecule modified with sulfur moiety as an anchor part to be adsorbed gold substrate. This was firstly reconstituted apo-protein solution. holo-protein then either bare substrate or pre-modified Native also same substrates for control experiment. The successfully pre-treated without denaturation structure, probably homogeneous anisotropic orientation. Guanidine hydrochloride buffer pH 5.0 applied remove from surface, thiolated derivative remaining surfaces. Apo-protein again introduced reconstitution holo-cyt.b562. success the...
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Eiji Itagaki, Graham Palmer, Lowell P. Hager, Studies on cytochrome b562 of Escherichia coli. II. Reconstitution of cytochrome b562 from apoprotein and hemin. Journal of Biological Chemistry. ,vol. 242, pp. 2272- 2277 ,(1966) , 10.1016/S0021-9258(18)96045-X
G F Ames, C Prody, S Kustu, Simple, rapid, and quantitative release of periplasmic proteins by chloroform. Journal of Bacteriology. ,vol. 160, pp. 1181- 1183 ,(1984) , 10.1128/JB.160.3.1181-1183.1984
P.C. Weber, F.R. Salemme, F.S. Mathews, P.H. Bethge, On the evolutionary relationship of the 4-alpha-helical heme proteins. The comparison of cytochrome b562 and cytochrome c'. Journal of Biological Chemistry. ,vol. 256, pp. 7702- 7704 ,(1981) , 10.1016/S0021-9258(18)43327-3
Eiji Itagaki, Lowell P. Hager, Studies on Cytochrome b562 of Escherichia coli I. PURIFICATION AND CRYSTALLIZATION OF CYTOCHROME b562 Journal of Biological Chemistry. ,vol. 241, pp. 3687- 3695 ,(1966) , 10.1016/S0021-9258(18)99827-3
Wolfgang Knoll, Polymer thin films and interfaces characterized with evanescent light Macromolecular Chemistry and Physics. ,vol. 192, pp. 2827- 2856 ,(1991) , 10.1002/MACP.1991.021921201
F.S. Mathews, P.H. Bethge, E.W. Czerwinski, The structure of cytochrome b562 from Escherichia coli at 2.5 A resolution. Journal of Biological Chemistry. ,vol. 254, pp. 1699- 1706 ,(1979) , 10.1016/S0021-9258(17)37829-8
Erwin Kretschmann, Die Bestimmung optischer Konstanten von Metallen durch Anregung von Oberflächenplasmaschwingungen European Physical Journal A. ,vol. 241, pp. 313- 324 ,(1971) , 10.1007/BF01395428
Katsuhiko Fujita, Masahiko Hara, Hiroyuki Sasabe, Wolfgang Knoll, Kazuma Tsuboi, Kotaro Kajikawa, Kazuhiko Seki, Yukio Ouchi, Solvatochromism of a merocyanine derivative in a self-assembled monolayer on gold substrates Langmuir. ,vol. 14, pp. 7456- 7462 ,(1998) , 10.1021/LA9806000
Taiji Furuno, Hiroyuki Sasabe, Akira Ikegami, Imaging two-dimensional arrays of soluble proteins by atomic force microscopy in contact mode using a sharp supertip Ultramicroscopy. ,vol. 70, pp. 125- 131 ,(1998) , 10.1016/S0304-3991(97)00103-4
Hun Gi Hong, Min Jiang, Stephen G. Sligar, Paul W. Bohn, Cysteine-specific surface tethering of genetically engineered cytochromes for fabrication of metalloprotein nanostructures Langmuir. ,vol. 10, pp. 153- 158 ,(1994) , 10.1021/LA00013A023