Cysteine-specific surface tethering of genetically engineered cytochromes for fabrication of metalloprotein nanostructures

摘要: The preparation of oriented metalloprotein nanostructures through introduction specific and complementary reactive groups on the solid protein surfaces is critically dependent reaction conditions used to prepare surface. Key problems include hydrolytic stability Si-O bond, low reactivity simple nucleophilic silane reagents, physisorption, identification for producing monolayer coverages. These are largely circumvented by utilizing a two-step linker synthesis, in which surface first prepared with (3-aminopropyl)silane (3-APS), resulting structure derivatized heterobifunctional reagent N-succinimidyl 6-maleimidocaproate (EMCS). maleimide functionality then presented protein, into single unique cysteine residue has been introduced genetic engineering techniques. Hydrolytic dramatically enhanced including postreaction curing step, temperature elevated drive alkoxylsilane condensation completion. Finally substituting gas-phase chemical vapor deposition procedure liquid-phase 3-APS produces better control over coverage quality films. 23 refs., 4 figs., 2 tabs.