Recent developments in the enzymatic O -glycosylation of flavonoids
作者： Bernd Hofer
DOI: 10.1007/S00253-016-7465-0
关键词:
摘要: The glycosylation of bioactive compounds, such as flavonoids, is particular relevance, it modulates many their pharmacokinetic parameters. This article reviews the literature between 2010 and end 2015 that deals with enzymatic O-glycosylation this class compounds. Enzymes glycosyltransferase family 1 remain biocatalysts choice for glycodiversification in spite relatively low yields. Transfers 14 different sugars, addition to glucose, were reported. Several Escherichia coli strains metabolically engineered enable a (more efficient) synthesis required donor during vivo glycosylations. For transfer enzymes glycoside hydrolase families 13 70 successfully assayed several flavonoids. number acceptor substrates regiospecificities characterized so far smaller than glycosyltransferases. However, glycosyl donors are much cheaper yields considerably higher. A few success stories enzyme engineering These improved catalytic efficiency well donor, acceptor, or product ranges. Currently, development appropriate high-throughput screening systems appears be major bottleneck powerful technology.
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Ramesh Prasad Pandey, Prakash Parajuli, Luan Luong Chu, Sumangala Darsandhari, Jae Kyung Sohng, Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli Biochemical Engineering Journal. ,vol. 101, pp. 191- 199 ,(2015) , 10.1016/J.BEJ.2015.05.017
Heike Overwin, Victor Wray, Bernd Hofer, Flavonoid glucosylation by non-Leloir glycosyltransferases: formation of multiple derivatives of 3,5,7,3′,4′-pentahydroxyflavane stereoisomers Applied Microbiology and Biotechnology. ,vol. 99, pp. 9565- 9576 ,(2015) , 10.1007/S00253-015-6760-5
Fei He, Wei-Kai Chen, Ke-Ji Yu, Xiang-Nan Ji, Chang-Qing Duan, Malcolm J. Reeves, Jun Wang, Molecular and biochemical characterization of the UDP-glucose: Anthocyanin 5-O-glucosyltransferase from Vitis amurensis Phytochemistry. ,vol. 117, pp. 363- 372 ,(2015) , 10.1016/J.PHYTOCHEM.2015.06.023
Vatsala Malik, Gary W. Black, Structural, functional, and mutagenesis studies of UDP-glycosyltransferases Advances in Protein Chemistry. ,vol. 87, pp. 87- 115 ,(2012) , 10.1016/B978-0-12-398312-1.00004-4
Heike Overwin, Victor Wray, Bernd Hofer, Biotransformation of phloretin by amylosucrase yields three novel dihydrochalcone glucosides. Journal of Biotechnology. ,vol. 211, pp. 103- 106 ,(2015) , 10.1016/J.JBIOTEC.2015.07.002
Hong-Mei Li, Jae Kyoung Lee, Li-Juan Nie, Qiang Huo, Tao Ma, Jae Kyung Sohng, Young-Soo Hong, Cheng-Zhu Wu, Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase Archives of Pharmacal Research. ,vol. 38, pp. 2208- 2215 ,(2015) , 10.1007/S12272-015-0658-8
Prakash Parajuli, Ramesh Prasad Pandey, Nguyen Thi Huyen Trang, Tae Jin Oh, Jae Kyung Sohng, Expanded acceptor substrates flexibility study of flavonol 7- O -rhamnosyltransferase, AtUGT89C1 from Arabidopsis thaliana Carbohydrate Research. ,vol. 418, pp. 13- 19 ,(2015) , 10.1016/J.CARRES.2015.09.010
Prakash Parajuli, Ramesh Prasad Pandey, Nguyen Thi Huyen Trang, Amit Kumar Chaudhary, Jae Kyung Sohng, Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli. Microbial Cell Factories. ,vol. 14, pp. 76- 76 ,(2015) , 10.1186/S12934-015-0261-1
Frederik De Bruyn, Maarten Van Brempt, Jo Maertens, Wouter Van Bellegem, Dries Duchi, Marjan De Mey, Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides. Microbial Cell Factories. ,vol. 14, pp. 138- 138 ,(2015) , 10.1186/S12934-015-0326-1
Jonathon Roepke, Gale G. Bozzo, Biocatalytic synthesis of quercetin 3-O-glucoside-7-O-rhamnoside by metabolic engineering of Escherichia coli. ChemBioChem. ,vol. 14, pp. 2418- 2422 ,(2013) , 10.1002/CBIC.201300474