Proteins interacting with Leishmania major PUF1: A proteomic dataset.
作者： África Sanchiz , Darío López-García , Carlos García-García , Irene Ozaez , Begoña Aguado
DOI: 10.1016/J.DIB.2020.106594
关键词:
摘要: Abstract Leishmania parasites must deal with stressful environmental conditions (thermal, nutritional and oxidative) along their digenetic life cycles. This requires drastic changes in gene expression, which this parasite occurs mainly through post-transcriptional mechanisms involving RNA binding proteins (RBPs). PUF proteins, a class of RBPs existing most eukaryotic organisms, might play too an essential role modulating the fate mRNAs regulating expression parasites. A proteomic approach to identify putative protein partners (interactome) major PUF1 was performed. The interactome characterized by co-immunoprecipitation using L. cellular extracts anti-LiPUF1 antibody, subsequent analysis co-immunoprecipitated mass-spectrometry, identifying 90 LmPUF1 candidate partners. Remarkably, many identified are other and/or P bodies mRNA-exporting machinery components. Raw mass spectrometry data available via ProteomeXchange identifier PXD022581.
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