IL-12 priming during in vitro antigenic stimulation changes properties of CD8 T cells and increases generation of effector and memory cells

摘要: Antigenic and costimulatory signals trigger a developmental program by which naive CD8 T cells differentiate into effector memory cells. However, initial cytokine that regulate the generation of are not well understood. In this study, we show IL-12 priming during in vitro antigenic stimulation results significant increase both primary cell population mice after adoptive transfer activated The effect is closely associated with qualitative changes cells, such as reduced MHC I tetramer binding CD69 expression, altered distribution lipid rafts, decreased cytolytic activity, less susceptibility to apoptosis. Furthermore, exogenous improved intrinsic survival properties leading better protective immunity vaccine-induced responses. experiments IL-12p40- IL-12Rβ1-deficient showed similar levels responses compared wild-type mice, implying endogenous and/or IL-12R signaling vivo critical for immunity. Together, our suggest can serve an important, but dispensable regulatory factor development could be useful many medical applications.