Biochemical detection of esterases in the adult female integument of organophosphate-resistant Boophilus microplus (Acari: Ixodidae).
作者： Mario A. Villarino , Suryakant D. Waghela , G. Gale Wagner
DOI: 10.1603/0022-2585-40.1.52
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摘要: Abstract Esterase activity was present in the integument of adult female Boophilus microplus (Canestrini) ticks that are resistant to organophosphates (OP). Three esterases were purified from integument, which hydrolyze substrates p-nitrophenylacetate and β-naphthyl acetate after comparison OP-resistant strain an OP-susceptible strains. The by ion-exchange chromatography characterized using different esterase inhibitors; eserine sulfate, diethyl p-nitrophenyl phosphate (paraoxon), para-hydroxyl-mercuribenzoate (pHMB), diisopropylphosphofluoridate (DFP). All had a molecular mass 64 Kd (PAGE), but based on inhibitor effects as B-esterase with affinity, carboxylesterase one A-Esterase (nonspecific esterase) affinity. described important detoxification mechanism B. tic...
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