The Vγ2/Vδ2 T‐cell repertoire in Macaca fascicularis: functional responses to phosphoantigen stimulation by the Vγ2/Jγ1.2 subset

摘要: Circulating Vgamma2/Vdelta2 T cells in human and non-human primates respond to small molecular weight non-peptidic phosphoantigens a major histocompatibility complex (MHC)-unrestricted manner. These responses are encoded by the Vgamma2/Jgamma1.2 chain of T-cell receptor positively selected during early development create biased repertoire adults. We characterized Vgamma2 cynomolgus macaques (Macaca fascicularis) develop primate model for studying effects infection therapy on circulating subset. The macaque was highly homologous from beings rhesus mulatta), though we noted conserved substitutions critical residues within CDR3 both species. Despite these substitutions, Vgamma2/Vdelta2+ monkeys exhibited polyclonal two different phosphoantigens. Proliferative were observed with isopentenylpyrophosphate alendronate, but stronger interferon-gamma secretory isopentenylpyrophosphate. In vitro stimulation expansion led selective outgrowth subset, marked shift spectratype. As result less starting Vgamma2, constitutes sensitive examining or vivo treatments population. Our studies establish value as antigens, further evidence remarkable evolutionary conservation this unusual, phosphoantigen-responsive subset that is found only