C-Type Natriuretic Peptide Specifically Acts on the Pylorus and Large Intestine in Mouse Gastrointestinal Tract

摘要: C-type natriuretic peptide (CNP) exerts its main biological effects by binding to receptor B (NPR-B), a membrane-bound guanylyl cyclase that produces cyclic guanosine monophosphate (cGMP). CNP is known cause gastrointestinal (GI) smooth muscle relaxation. Experimental evidence suggests connection between signaling and GI function, with reactive regions in the tract possibly affecting transit; however, this relation has not yet been conclusively shown. Here, we show plays important region-specific roles of mice. We found treatment (1 or 2 mg/kg) increased transient cGMP production pylorus, colon, rectum, higher dose (2 enhancing gastric emptying mice; increase levels was however absent NPR-B-deficient short-limbed dwarfism (SLW) mouse. Furthermore, NPR-B highly expressed being localized nerve fibers nuclei cytoplasm cells blood vessels. Our in vivo findings showed NPR-B-mediated after administration specifically acted on rectum contributed emptying. may thus be potential therapeutic agent for motility/transit disorders such as ileus pyloric stenosis.