Induced pluripotency enables differentiation of human nullipotent embryonal carcinoma cells N2102Ep.

摘要: Embryonal carcinoma (EC) cells, which are considered to be malignant counterparts of embryonic stem comprise the pluripotent cell component teratocarcinomas, a form testicular germ tumors (GCTs). Nevertheless, many established human EC lines nullipotent with limited or no capacity differentiate under normal circumstances. In this study, we tested whether an over-expression Yamanaka's reprogramming factors OCT4, SOX2, c-MYC and KLF4 might enable differentiation cells N2102Ep. Using OCT4 knockdown differentiated N2102Ep able derive reprogrammed lines. The induced pluripotency allows toward neural lineage by retinoic acid; expression SSEA3 SSEA4 is down-regulated, whereas that surface markers up-regulated. Consistent up-regulation markers, master neuroectodermal transcription factor PAX6 also in We next investigated induce spontaneous However, while ectopic promotes NTERA2, it induces death nevertheless find upon induction acid, mature neuronal morphology similar NTERA2 as determined TUJ1 expression, absent parental cells. Altogether, conclude state can acquire more relaxed potential factors.