Direct injection analysis of chlorzoxazone and its major metabolite 6-hydroxychlorzoxazone in human serum using a semipermeable surface (SPS) HPLC column.
作者： A. Haque , J. T. Stewart
DOI: 10.1002/(SICI)1099-0801(199707)11:4<236::AID-BMC679>3.0.CO;2-I
关键词:
摘要: A semipermeable surface (SPS) HPLC column has been used to develop a direct injection method for the simultaneous determination of chlorzoxazone and its 6-hydroxy metabolite in serum. Spiked serum was filtered 20 microL injected directly onto SPS column. The separation achieved within 25 min using mobile phase 20:80 v/v acetonitrile-aqueous 0.05 M phosphate buffer pH 7 with detection at 230 nm. linear concentration range 0.5-20 micrograms/mL (r > 0.999, n = 6) an RSD 3), respectively, both drug metabolite. Intraday interday reproducibility 94%.
sci-hub.se 参考文章(13)
Catherine Girre, Danièle Lucas, Eric Hispard, Catherine Menez, Sylvain Dally, Jean-François Menez, Assessment of cytochrome P4502E1 induction in alcoholic patients by chlorzoxazone pharmacokinetics. Biochemical Pharmacology. ,vol. 47, pp. 1503- 1508 ,(1994) , 10.1016/0006-2952(94)90524-X
Carla P. Desilets, Mary Ann Rounds, Fred E. Regnier, Semipermeable-surface reversed-phase media for high-performance liquid chromatography. Journal of Chromatography A. ,vol. 544, pp. 25- 39 ,(1991) , 10.1016/S0021-9673(01)83976-5
B.J. Gurley, S. Zermatten, D. Skelton, Determination of antipyrine in human serum by direct injection restricted access media liquid chromatography Journal of Pharmaceutical and Biomedical Analysis. ,vol. 12, pp. 1591- 1595 ,(1994) , 10.1016/0731-7085(94)00101-4
Jingyi He, Akimasa Shibukawa, Terumichi Nakagawa, Direct injection analysis of carbamazepine and its active 10,11-epoxide metabolite in plasma by use of a semipermeable surface (SPS) silica column in LC. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 10, pp. 289- 294 ,(1992) , 10.1016/0731-7085(92)80041-K
Thomas C. Pinkerton, High-performance liquid chromatography packing materials for the analysis of small molecules in biological matrices by direct injection. Journal of Chromatography A. ,vol. 544, pp. 13- 23 ,(1991) , 10.1016/S0021-9673(01)83975-3
James T. Stewart, Che W. Chan, Fluorometric Determination of Chlorzoxazone in Tablets and Biological Fluids Journal of Pharmaceutical Sciences. ,vol. 68, pp. 910- 912 ,(1979) , 10.1002/JPS.2600680735
Steven H.Y. Wong, Advances in liquid chromatography and related methodologies for therapeutic drug monitoring. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 7, pp. 1011- 1032 ,(1989) , 10.1016/0731-7085(89)80041-X
Daniele Lucas, Francois Berthou, Yvonne Dreano, Patrick Lozac'h, Alain Volant, Jean-Francois Menez, Comparison of Levels of Cytochromes P‐450, CYP1A2, CYP2E1, and Their Related Monooxygenase Activities in Human Surgical Liver Samples Alcoholism: Clinical and Experimental Research. ,vol. 17, pp. 900- 905 ,(1993) , 10.1111/J.1530-0277.1993.TB00861.X
Hailang Zhang, James T. Stewart, Improved High Performance Liquid Chromatographic Determination of Chlorzoxazone and its Hydroxy Metabolite in Human Serum and Urine Analytical Letters. ,vol. 26, pp. 675- 687 ,(1993) , 10.1080/00032719308017400
Raimund Peter, Ronald Boecker, Philippe H. Beaune, Masahiko Iwasaki, F. Peter Guengerich, Chung S. Yang, Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1 Chemical Research in Toxicology. ,vol. 3, pp. 566- 573 ,(1990) , 10.1021/TX00018A012