The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei

摘要: Post-transcriptional gene regulation is essential to eukaryotic development. This particularly emphasized in trypanosome parasites where genes are co-transcribed polycistronic arrays but not necessarily co-regulated. The small CCCH protein, TbZFP3, has been identified as a trans-acting post-transcriptional regulator of Procyclin surface antigen expression Trypanosoma brucei. To investigate the wider role TbZFP3 parasite transmission, global analysis associating transcripts was carried out. Examination subset selected revealed their increased abundance through mRNA stabilization upon ectopic overexpression, dependent integrity zinc finger domain. Reporter assays demonstrated that this mediated 3′-UTR sequences for two target transcripts. Global developmental profiling cohort TbZFP3-selected significant enrichment transmissible stumpy forms parasite. specific mRNAs by TbZFP3mRNP provides evidence regulon with potential co-ordinate important transmission.