A surface transporter family conveys the trypanosome differentiation signal.
作者： Samuel Dean , Rosa Marchetti , Kiaran Kirk , Keith R. Matthews
DOI: 10.1038/NATURE07997
关键词:
摘要: Microbial pathogens use environmental cues to trigger the developmental events needed infect mammalian hosts or transmit disease vectors. The parasites causing African sleeping sickness respond citrate cis-aconitate (CCA) initiate life-cycle development when transmitted their tsetse fly vector. This requires hypersensitization of CCA by exposure low temperature, conditions encountered after feeding at dusk dawn. Here we identify a carboxylate-transporter family, PAD (proteins associated with differentiation), required for perception this differentiation signal. Consistent predictions response trypanosomes CCA, proteins are expressed on surface transmission-competent 'stumpy-form' in bloodstream, and least one member is thermoregulated, showing elevated expression access temperature. Moreover, RNA-interference-mediated ablation diminishes CCA-induced eliminates hypersensitivity under cold-shock conditions. As well as being molecular transducers signal these parasites, provide first example marker able discriminate transmission stage host.
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