A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes1Note: Nucleotide sequences data reported in this paper are available in the EMBL, GenBank™ and DDJB data bases under the accession number X99114.1
作者： Jose M Requena , Manuel Soto , Luis Quijada , Graciela Carrillo , Carlos Alonso
DOI: 10.1016/S0166-6851(96)02785-5
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摘要: Abstract A novel repetitive DNA element has been isolated from the Leishmania infantum genome. The 348 bp long element, designated LiR3, was found to be located downstream 3'-end of ribosomal RNA (rRNA) genes. This LiR3 short sequences with potential form stem-loop structures similar those bacterial rho-independent transcriptional terminators. Given both structural features and genomic location this we searched for a possible functional implication these in termination rRNA transcription. Nuclear run-on assays indicated that indeed there is blockage associated element. Several χ -like elements, resembling recombination-promoting sites Escherichia coli , were identified within structures. elements gene conversion events discussed.
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sci-hub.se 参考文章(34)
David F. Spencer, James C. Collings, Murray N. Schnare, Michael W. Gray, Multiple spacer sequences in the nuclear large subunit ribosomal RNA gene of Crithidia fasciculata The EMBO Journal. ,vol. 6, pp. 1063- 1071 ,(1987) , 10.1002/J.1460-2075.1987.TB04859.X
G. Kendall, A. F. Wilderspin, F. Ashall, M. A. Miles, J. M. Kelly, Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase does not conform to the 'hotspot' topogenic signal model. The EMBO Journal. ,vol. 9, pp. 2751- 2758 ,(1990) , 10.1002/J.1460-2075.1990.TB07462.X
Manuel Soto, Jose M Requena, M Garcia, LC Gomez, I Navarrete, C Alonso, None, Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania. Journal of Biological Chemistry. ,vol. 268, pp. 21835- 21843 ,(1993) , 10.1016/S0021-9258(20)80617-6
Sheryl Henderson, Barbara Sollner-Webb, A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene. Cell. ,vol. 47, pp. 891- 900 ,(1986) , 10.1016/0092-8674(86)90804-4
Theodore C. White, Gloria Rudenko, Piet Borst, Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs Nucleic Acids Research. ,vol. 14, pp. 9471- 9489 ,(1986) , 10.1093/NAR/14.23.9471
P. Dietrich, M.B. Soares, M.H.T. Affonso, L.M. Floeter-Winter, The Trypanosoma cruzi ribosomal RNA-encoding gene: analysis of promoter and upstream intergenic spacer sequences. Gene. ,vol. 125, pp. 103- 107 ,(1993) , 10.1016/0378-1119(93)90753-P
Ernst J.M. Grondal, Raymond Evers, Albert W.C.A. Cornelissen, Identification and sequence analysis of the ribosomal DNA promoter region of Crithidia fasciculata Nucleic Acids Research. ,vol. 18, pp. 1333- 1338 ,(1990) , 10.1093/NAR/18.6.1333
Doug Looker, Laurel A. Miller, Hille J. Elwood, Shawn Stickel, Mitchell L. Sogin, Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region. Nucleic Acids Research. ,vol. 16, pp. 7198- 7198 ,(1988) , 10.1093/NAR/16.14.7198
Palmira Guevara, Guillermina Alonso, JoséFranco da Silveira, Maricilda de Mello, JoséVicente Scorza, Néstor Añez, JoséLuis Ramírez, None, Identification of new world Leishmania using ribosomal gene spacer probes Molecular and Biochemical Parasitology. ,vol. 56, pp. 15- 26 ,(1992) , 10.1016/0166-6851(92)90150-I
Estela M. Novak, Maricilda P. de Mello, Henrique B.M. Gomes, Ivan Galindo, Palmira Guevara, JoséL. Ramirez, JoséFranco da Silveira, Repetitive sequences in the ribosomal intergenic spacer of Trypanosoma cruzi Molecular and Biochemical Parasitology. ,vol. 60, pp. 273- 280 ,(1993) , 10.1016/0166-6851(93)90138-N