A new Agrobacterium strain isolated from aerial tumors on Ficus benjamina L.

摘要: Crown gall tumors, collected from branches of 1-year-old weeping fig (Ficus benjamina L.) trees, yielded both tumorigenic and nonpathogenic agrobacteria. On the basis classical diagnostic tests, strains were identified as Agrobacterium tumefaciens, whereas could not be assigned to any known terrestrial spp. The also differed other members genus by producing more acid mannitol. According cluster analysis carbon substrate oxidation (GN Microplate; Biolog, Inc.) fatty content, distinct A. rhizogenes, vitis, rubi. Furthermore, they had unusual opine metabolism, inducing tumors that synthesized nopaline three recently discovered opines: chrysopine (d-lactone N-1-deoxy-D-fructosyl-L-glutamine, N-1-deoxy-D-fructosyl-5-oxo-L-proline. tumefaciens present in same unable degrade opines tested. phylogenetic position strain AF3.10 was inferred comparing its rrs (i.e., 16S rRNA gene) sequence with those type Rhizobium species. showed clustered rubi but vitis far removed rhizogenes. However, significantly different suggest may a new species is these two are one another.