Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer.
作者： Elisabeth Navarro , Pascal Simonet , Philippe Normand , René Bardin
DOI: 10.1007/BF00245277
关键词:
摘要: DNA sequences from the intergenic spacer (IGS) region of ribosomal operon were amplified by polymerase chain reaction (PCR) technique using two primers derived 16S and 23S rRNA conserved sequences. The PCR products, cleaved 4 base cutting restriction enzymes, used to differentiate Nitrobacter strains. This method offered a convenient alternative serological testing for characterization isolates enabled large number strains be genotypically characterized easily rapidly. was successfully characterize natural populations various soils lake. A diversity demonstrated in soils, lake both freshwater sediments. Strains closely related WL LL found these eco-systems. It seems that not associated with global environments but may presence locally coexisting niches.
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