Evidence of increased angiogenesis in patients with acute myeloid leukemia

摘要: Angiogenesis plays a key role in solid tumor growth. The purpose of this work was to study angiogenesis acute myeloid leukemia (AML). We stained bone marrow samples from 20 adult patients with untreated AML and normal controls using endothelial cell markers (ULEX-E von Willebrand factor [vWF]). number vessels per millimeter length core biopsy specimen scored by light microscopy. Using ULEX-E staining, marrows had (average +/- SEM) 8.3 3.6 vessels/mm (range, 3.7-19.3), whereas 4.3 1.8 1.6-7.9). A similar difference noted vWF staining (8.6 3.0 vs 4. 9 2.2 marrows, respectively). differences between the numbers were highly significant (P <.0001 for both staining). When analyzed FAB category, there no average among different subgroups AML. reverse transcriptase polymerase chain reaction, we observed that HL-60 U937 human lines 4 freshly isolated cells expressed mRNA vascular growth (VEGF). Both as well all fresh isolates tested VEGF protein. Basic fibroblast only 3 samples. These observations suggest may play pathogenesis Inhibition could constitute novel strategy treatment (Blood. 2000;95:309-313).