Hormonal regulation of some steps of thyroglobulin synthesis and secretion in bicameral cell culture.

摘要: Porcine thyroid cells were cultured for 15 days on porous bottom chambers with or without different mixtures of hormones added to serum-free basal medium. Assays 10% serum also performed comparison previously published results. The effects the hormones, particularly insulin, TSH and hydrocortisone, studied total RNA content, thyroglobulin mRNA level, amount secreted into apical medium glycosylation. Insulin similarly increased their additive. Thyroglobulin content was twofold by insulin threefold TSH. When they simultaneously, maximal level reached, showing that gene expression Hydrocortisone alone did not modify but hormone amplified when present together. fourfold these two together appeared be had no effect even combined However, three together, hormonal response amplified. incorporation 3H-mannose as well anionic residue molecule © 1994 Wiley-Liss, Inc.