Influence of collagen gel on the orientation of epithelial cell polarity: follicle formation from isolated thyroid cells and from preformed monolayers.
作者： M Chambard , J Gabrion , J Mauchamp
DOI: 10.1083/JCB.91.1.157
关键词:
摘要: The influence of collagen gels on the orientation polarity epithelial thyroid cells in culture was studied under four different conditions. (a) Isolated cultured surface a gel formed monolayer. apical pole contact with medium and basal membrane attached to substratum. (b) embedded inside organized within 8 into follicles. oriented towards interior follicular lumen. (c) Cells were first floating vesicles, structures which is medium, vesicles gel. After 3 d, cell inverted, being cavity encompassed by cells. Vesicles had been transformed (d) Monolayers as overlaid second layer collagen, polymerized surface. A disorganization continuous pavement occurred 24 h; upper reorganized follicles sandwich 4-8 d. similar process when monolayer grown plastic or covered small pieces glass cover slips. No reorganization observed between two surfaces. In conclusion, first, always area an adhesive and, second, interaction preformed not compatible stability this domain plasma membrane. extracellular components having properties appears be determinant factor stabilization polarity.
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Henry C. Pitot, Carol A. Sattler, Gerald L. Sattler, George Michalopoulos, Ultrastructure of adult rat hepatocytes cultured on floating collagen membranes. Cancer Research. ,vol. 38, pp. 1539- 1549 ,(1978)
Frederick Grinnell, Cellular adhesiveness and extracellular substrata. International Review of Cytology-a Survey of Cell Biology. ,vol. 53, pp. 65- 144 ,(1978) , 10.1016/S0074-7696(08)62241-X
J. Yang, J. Richards, P. Bowman, R. Guzman, J. Enami, K. McCormick, S. Hamamoto, D. Pitelka, S. Nandi, Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 3401- 3405 ,(1979) , 10.1073/PNAS.76.7.3401
D. C. Bennett, Morphogenesis of branching tubules in cultures of cloned mammary epithelial cells. Nature. ,vol. 285, pp. 657- 659 ,(1980) , 10.1038/285657A0
KINJI INOUE, RYUYA HORIUCHI, YOICHI KONDO, Effect of Thyrotropin on Cell Orientation and Follicle Reconstruction in Rotated Suspension Culture of Hog Thyroid Cells Endocrinology. ,vol. 107, pp. 1162- 1168 ,(1980) , 10.1210/ENDO-107-4-1162
Ü.Hoi Sang, M.H. Saier, M.H. Ellisman, Tight junction formation is closely linked to the polar redistribution of intramembranous particles in aggregating MDCK epithelia Experimental Cell Research. ,vol. 122, pp. 384- 391 ,(1979) , 10.1016/0014-4827(79)90315-X
Tom Elsdale, Jonathan Bard, CELLULAR INTERACTIONS IN MORPHOGENESIS OF EPITHELIAL MESENCHYMAL SYSTEMS Journal of Cell Biology. ,vol. 63, pp. 343- 349 ,(1974) , 10.1083/JCB.63.1.343
PIETRO DE CAMILLI, DIONISIO PELUCHETTI, JACOPO MELDOLESI, Structural difference between luminal and lateral plasmalemma in pancreatic acinar cells. Nature. ,vol. 248, pp. 245- 247 ,(1974) , 10.1038/248245B0
H K Kleinman, R J Klebe, G R Martin, Role of collagenous matrices in the adhesion and growth of cells. Journal of Cell Biology. ,vol. 88, pp. 473- 485 ,(1981) , 10.1083/JCB.88.3.473
Odile Chabaud, Judith Chebath, Annie Giraud, Jean Mauchamp, Modulation by thyrotropin of thyroglobulin synthesis in cultured thyroid cells : Correlations with polysome profile and cytoplasmic thyroglobulin mRNA content Biochemical and Biophysical Research Communications. ,vol. 93, pp. 118- 126 ,(1980) , 10.1016/S0006-291X(80)80254-3