Substrate modulation of cultured rabbit conjunctival epithelial cell differentiation and morphology.

摘要: This study demonstrated that growth and differentiation of rabbit conjunctival epithelial cells could be promoted by such substrata as collagen gel, matrigel or various mixtures in a defined culture system. On conventional plastic glass culture, the adopted monolayer small epithelioid primary culture. They became enlarged, squamoid exhibited notable senescence upon subcultures. formed an organized sheet with cuboid shape cell polarity. matrigel, globules stratified appearance including basal layer outer part globule central globule. The origin these cultures was verified positive immunostaining anti-keratin monoclonal antibodies. Expression mucin antigen lost on but gel staining periodic acid Schiff's reagent anti-mucin antibody stainings. These results indicate both can provide permissive substrate environment for goblet differentiation. Furthermore, this unique phenotypic expression may possessed only selective subpopulation. system will allow us to further explore mechanism which is controlled. Invest Ophthalmol Vis Sci 29:1565-1576,1988 ocular surface covered corneal epithelia. Conjunctiva contains mucin-producing function are crucial integrity. Conjunctival proliferation altered number diseases, resulting squamous metaplasia, 1 ' 2 manifested deficiency secondary loss keratinization. In order investigate pathogenesis we have understand normal differentiation, especially development vitro would important regard. Previously, researchers found limited when substra