Studies of Adenovirus-Induced Eye Disease in the Rabbit Model

摘要: Purpose. To achieve a better understanding of the pathogenic processes associated with human adenovirus (Ad)-induced ocular disease. Methods. Growth curves Ad5 and Ad14 were performed in cell cultures derived from rabbit corneal epithelium (CE) keratocytes (CK). For vivo studies, eyes inoculated intrastromally topically 10 6 plaque-forming units per eye ultraviolet light-inactivated (UV-I) or Ad14, clinical features evaluated by biomicroscopic slit lamp examinations. Duration quantitation virus tear samples monitored. Humoral response was enzyme-linked immunosorbent assay serum neutralization titrations. Histopathologic immunocytochemical staining frozen tissues to determine expression major histocompatibility complex (MHC) class I II presence CD4 + CD8 T lymphocytes CD18 cells after immunopathologic elicited inoculation. Results. Both replicated all studied. In origin, cultured CE CK cells, whereas replication appeared restricted. Virus titers Ad5-inoculated peaked on postinoculation days 3 through 4, approximately 100-fold increase infectious comparison initial titers. The duration shedding 8.9 ± 2.4 days. Ad5, UV-I, induced seroconversion subepithelial opacities. present these intrastromal immune infiltrates. Expression MHC observed ; also expressed inflamed areas. Conclusions. is capable replicating both eye. Ad antigens within stroma originating (Ad5), UV-inactivated nonreplicating (Ad14) can elicit indistinguishable responses composed , cells.