Deciphering of the Human Interferon-Regulated Proteome by Mass Spectrometry-Based Quantitative Analysis Reveals Extent and Dynamics of Protein Induction and Repression

摘要: Interferons (IFNs) are pleotropic cytokines secreted upon encounter of pathogens and tumors. Applying their antipathogenic, antiproliferative, immune stimulatory capacities, recombinant IFNs frequently prescribed as drugs to treat different diseases. act by changing the gene expression profile cells. Due characteristics such rapid induction signaling, also represent prototypical model systems for various aspects biomedical research (e.g., signal transduction). In regard signaling activated promoters, can be subdivided into two groups. Here, alterations cellular proteome human cells treated with IFNα IFNγ were elucidated in a time-resolved manner quantitative analysis. The majority protein regulations strongly IFN type time dependent. addition expected upregulation IFN-responsive proteins, an astonishing number proteins became profoundly repressed especially IFNγ. Thus, our comprehensive analysis revealed important insights IFN-regulated its dynamics repression. Interestingly, new class IFN-repressed genes comprises known host factors highly relevant HIV, dengue virus, hepatitis C virus.