Zinc Binding to MG53 Protein Facilitates Repair of Injury to Cell Membranes

摘要: Zinc is an essential trace element that participates in a wide range of biological functions, including wound healing. Although Zn(2+) deficiency has been linked to compromised healing and tissue repair human diseases, the molecular mechanisms underlying Zn(2+)-mediated remain unknown. Our previous studies established MG53, TRIM (tripartite motif) family protein, component cell membrane machinery. Domain homology analysis revealed MG53 contains two Zn(2+)-binding motifs. Here, we show binding indispensable assembly Live imaging illustrated entry from extracellular space for translocation MG53-containing vesicles acute injury sites formation patch. The effect on abolished mg53(-/-) muscle fibers, suggesting functions as potential target during repair. Mutagenesis suggested both RING B-box motifs constitute domains contribute MG53-mediated Overall, this study establishes base interaction with protection against membrane.