Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease.

摘要: Retinitis pigmentosa (RP) is an inherited retinal disease characterized by progressive loss of photoreceptor cells. This study aim at exploring the effect pigment epithelium (RPE) derived from human-induced pluripotent stem cell (hiPSC-RPE) on retina degeneration 10 (rd10) mice, which are with death. We generated RPE hiPSCs sequential supplementation retinal-inducing factors and specification signaling factors. The three-dimensional (3D) spheroid culture method was used to obtain optimal injectable hiPSC-RPE Subretinal space transplantation conducted deliver cells into rd10 mice. Neurotrophic factor secretion transplanted detected enzyme-linked immunosorbent assay (ELISA). Immunostaining, Western blotting, electroretinography (ERG), visual behavior testing were performed determine effects function in Our data demonstrated that exhibited classic properties phenotype after induction hiPSCs. co-cultured mouse explants or ganglion 5 (RGC5) decreased apoptosis. viability functional enhanced 3D culture. Transplanted hiPSC-derived identified immunostaining human nuclear antigen staining 14 days subretinal injection. epithelium-derived level increased significantly. expression CD68, microglial activation marker, reduced transplantation. light avoidance ERG mice improved findings suggest can rescue structure photoreceptors sub-retinal transplantation, lay foundation for future clinical therapy treat RP other diseases.