From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium

摘要: Progress in retinal-cell therapy derived from human pluripotent stem cells currently faces technical challenges that require the development of easy and standardized protocols. Here, we developed a simple retinal differentiation method, based on confluent induced (hiPSC), bypassing embryoid body formation use exogenous molecules, coating, or Matrigel. In 2 wk, generated both pigmented epithelial self-forming neural retina (NR)-like structures containing progenitor (RPCs). We report sequential RPCs to seven neuroretinal cell types maturated NR-like as floating cultures, thereby revealing multipotency integration-free hiPSCs. Furthermore, Notch pathway inhibition boosted generation photoreceptor precursor cells, crucial establishing strategies. This innovative process proposed here provides readily efficient scalable approach produce for regenerative medicine drug-screening purposes, well an vitro model disease.