Postvasectomy alterations in protein synthesis and secretion in the rat caput epididymidis are not repaired after vasovasostomy.

摘要: Many men who have undergone vasectomy later request vasovasostomy. Unfortunately, significant numbers of these remain infertile despite the reestablishment patent ducts. This report examines possibility that epididymal function remains compromised after vasovasostomy in rat by examination quantifiable, vivo protein synthesis and secretion caput epididymidis. Rats were studied 30 days vasectomy, a (which was performed vasectomy), or sham operations. Epididymal lumen fluids (LF) collected micropuncture 3 hours' microperifusion tubules with 35 S-amino acids. Proteins separated 2-dimensional electrophoresis detected Coomassie blue staining. Synthesized proteins tubule extract synthesized secreted LF autoradiography image analysis. Specific appeared to be affected vasectomy-vasovasostomy identified internal sequence contained an average 87 detectable control caput. Nineteen most prominent selected for more focused study. The clusterin, cysteine-rich secretory (CRISP)-1, retinoic acid-binding protein. Among these, CRISP-1 remained reduced Two minor as prostaglandin D 2 synthase phosphatidylethanolamine-binding All occur epididymides multiple species been associated sperm fertilizing capacity.