Monoclonal antibodies to epididymis-specific proteins using mice rendered immune tolerant to testicular proteins.

摘要: Monoclonal antibodies (mabs) have been used as a powerful tool for identification of newer sperm proteins. However, conventional hybridoma technology rarely provides chance to obtain mabs epididymal To increase this chance, we an alternate method neonatal tolerization. In protocol, animals were tolerized at birth using testicular proteins followed by immunization with cauda protein (which is cocktail both from and origin). This protocol induced specific immune response Spleen one these was then preparation mabs. fusion resulted in number reacting specifically Although identified approximately similar molecular weight on 1-dimensional Western blot analysis, there differences regional localization rat seen indirect immunofluorescence. Immunohistochemical epididymis showed region synthesis. The synthesis the distal caput epididymis, maximum expression supranuclear corpus epithelium. localized region. Epididymis agglutinating nature underlines functional importance maturation epididymis. These could therefore, be tools understanding physiology role fertilization.