Generation of clinical-grade red blood cells from human umbilical cord blood mononuclear cells

摘要: A xeno-free method for ex vivo generation of red blood cells (RBCs) is attempted in order to replicate large-scale production and clinical applications. An efficient milieu was formulated using injectable drugs substituting the animal-derived components culture medium. Unfractionated mononuclear isolated from human umbilical cord were used hypothesizing that heterogeneous cell population could effectively contribute erythroid generation. The strategy adopted includes a combination erythropoietin other under low oxygen levels, which resulted an increase number mature RBCs produced vitro. novelty this study addition supplements medium stage-specific manner differentiation unfractionated (MNCs) into erythropoietic lineage. lineage well established by day 21, wherein mean count found be 21.36 ± 0.9 × 108 further confirmed upregulated expression CD235a+ specific RBCs. rationale have simple produce isolates vitro mitigating effects multiple erythroid-activating agents batch variability.